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dc.contributor.advisorDharmasiri, Sunethra
dc.contributor.authorSanchez, Elizabeth ( )
dc.date.accessioned2020-06-16T20:21:10Z
dc.date.available2020-06-16T20:21:10Z
dc.date.issued2019-12
dc.identifier.citationSanchez, E. (2019). Characterization of the role of PIC30 protein in plant immune response (Unpublished thesis). Texas State University, San Marcos, Texas.
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/11836
dc.descriptionPresented to the Honors Committee of Texas State University in Partial Fulfillment of the Requirements for Graduation in the University Honors Program, December 2019.
dc.description.abstractPlants have developed several defense mechanisms to cope with biotic and abiotic stresses, including hypersensitive response (HR), an apoptotic response of plant tissues to infecting microbes leading to systemic acquired resistance (SAR). Salicylic acid plays an important role in plants when sending signals from the sites of HR to healthy plant organs during the development of SAR. PIC30, a protein previously characterized as a transporter protein belonging to the Major Facilitator Superfamily (MFS) in the model plant Arabidopsis thaliana, transports nitrate ions and the synthetic auxin, picloram into cells. A transgenic reporter line PIC30::PIC30-GUS displayed increased expression of the fusion protein when plants were exposed to salicylic acid. When salicylic acid binds to its receptor, NPR1, copper must also bind to NPR1 to facilitate the interaction. Therefore, copper is required for the activation of defense genes during salicylic acid-mediated SAR. PIC30::PIC3-GUS transgenic line also displayed a response to salicylic acid and copper. Primary root elongation of wild-type plants was inhibited while the roots of pic30 knockout mutant displayed slight resistance to high salicylic acid and copper concentrations. Overexpression lines of pic30 displayed high inhibition suggesting a possible role of PIC30 in the transport of salicylic acid and copper ions during SAR in plants.en_US
dc.formatText
dc.format.extent25 pages
dc.format.medium1 file (.pdf)
dc.language.isoen_USen_US
dc.subjectPIC30en_US
dc.subjectTransport proteinen_US
dc.subjectSystemic acquired resistanceen_US
dc.subjectPlant immune responseen_US
dc.titleCharacterization of the Role of PIC30 Protein in Plant Immune Responseen_US
txstate.documenttypeThesis
dc.contributor.committeeMemberDharmasiri, Nihal
thesis.degree.departmentHonors College
thesis.degree.disciplineBiology
thesis.degree.grantorTexas State University
txstate.departmentHonors College


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