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dc.contributor.advisorRodriguez, David
dc.contributor.authorBardan Sarmiento, Mario ( )
dc.date.accessioned2020-06-17T15:10:12Z
dc.date.available2020-06-17T15:10:12Z
dc.date.issued2019-05
dc.identifier.citationBardan Sarmiento, M. (2019). Magnetic capture hybridization of amphibian mitochondrial genomes (Unpublished thesis). Texas State University, San Marcos, Texas.
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/11841
dc.description.abstractAnalysis of mitochondrial DNA (mtDNA) provides useful insights into population dynamics of target organisms, and efficient extraction of this DNA is pivotal to ensuring the reliability of subsequent reactions. When extracted from organic tissue, resulting solutions contain a mixture of genomic and mitochondrial DNA, and separation of these two molecules usually requires the use of an ultracentrifuge; because many amphibian organisms of interest are found in tropical regions of the world without ready access to the required machinery, a new, more portable, method of selectively isolating mitochondrial genomes from complex mixtures to facilitate whole mitochondrial genome sequencing is proposed here. Conserved sequences in the 12S and 16S regions can be targeted by a uniquely designed biotinylated probe that will form a complex with magnetic beads covalently coupled to streptavidin. This research shows that the complex can be selectively filtered from solution through magnetic capture, and heat treatment then dissociates the probe complex from the mtDNA. The resulting solution contains samples suitable for downstream reactions to expand the knowledge of population dynamics as they relate to the emerging threat of chytridiomycosis in Anuran populations.en_US
dc.formatText
dc.format.extent35 pages
dc.format.medium1 file (.pdf)
dc.language.isoen
dc.subjectAmphibianen_US
dc.subjectMagnetic capture hybridizationen_US
dc.subjectMitochondrial genomeen_US
dc.subjectDNA extractionen_US
dc.subjectPortableen_US
dc.titleMagnetic Capture Hybridization of Amphibian Mitochondrial Genomesen_US
txstate.documenttypeThesis
thesis.degree.departmentHonors College
thesis.degree.disciplineBiology
thesis.degree.grantorTexas State University
dc.description.departmentHonors College


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