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dc.contributor.authorDaley, James M. ( Orcid Icon 0000-0002-7706-6134 )
dc.contributor.authorTomimatsu, Nozomi ( )
dc.contributor.authorHooks, Grace ( )
dc.contributor.authorWang, Weibin ( )
dc.contributor.authorMiller, Adam S. ( )
dc.contributor.authorXue, Xiaoyu ( )
dc.contributor.authorNguyen, Kevin A. ( )
dc.contributor.authorKaur, Hardeep ( )
dc.contributor.authorWilliamson, Elizabeth ( )
dc.contributor.authorMukherjee, Bipasha ( )
dc.contributor.authorHromas, Robert ( Orcid Icon 0000-0002-1916-6276 )
dc.contributor.authorBurma, Sandeep ( Orcid Icon 0000-0001-7057-9845 )
dc.contributor.authorSung, Patrick ( Orcid Icon 0000-0003-1396-9040 )
dc.date.accessioned2020-07-22T13:58:06Z
dc.date.available2020-07-22T13:58:06Z
dc.date.issued2020-06
dc.identifier.citationDaley, J. M., Tomimatsu, N., Hooks, G., Wang, W., Miller, A. S., Xue, X., Nguyen, K. A., Kaur, H., Williamson, E., Mukherjee, B., Hromas, R., Burma, S., & Sung, P. (2020). Specificity of end resection pathways for double-strand break regions containing ribonucleotides and base lesions. Nature Communications, 11(1), pp. 1-12.en_US
dc.identifier.issn2041-1723
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/12138
dc.description.abstractDNA double-strand break repair by homologous recombination begins with nucleolytic resection of the 5’ DNA strand at the break ends. Long-range resection is catalyzed by EXO1 and BLM-DNA2, which likely have to navigate through ribonucleotides and damaged bases. Here, we show that a short stretch of ribonucleotides at the 5’ terminus stimulates resection by EXO1. Ribonucleotides within a 5’ flap are resistant to cleavage by DNA2, and extended RNA:DNA hybrids inhibit both strand separation by BLM and resection by EXO1. Moreover, 8-oxo-guanine impedes EXO1 but enhances resection by BLM-DNA2, and an apurinic/ apyrimidinic site stimulates resection by BLM-DNA2 and DNA strand unwinding by BLM. Accordingly, depletion of OGG1 or APE1 leads to greater dependence of DNA resection on DNA2. Importantly, RNase H2A deficiency impairs resection overall, which we attribute to the accumulation of long RNA:DNA hybrids at DNA ends. Our results help explain why eukaryotic cells possess multiple resection nucleases.
dc.formatText
dc.format.extent13 pages
dc.format.medium1 file (.pdf)
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.sourceNature Communications, 2020, Vol. 11, No. 1, pp. 1-12.
dc.subjectDNA
dc.subjectDouble-strand break repair
dc.subjectRibonucleotides
dc.titleSpecificity of End Resection Pathways for Double-Strand Break Regions Containing Ribonucleotides and Base Lesionsen_US
txstate.documenttypeArticle
dc.rights.holder© The Author(s) 2020.
dc.identifier.doihttps://doi.org/10.1038/s41467-020-16903-4
dc.rights.licenseCreative Commons License
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
txstate.departmentChemistry and Biochemistry


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