Identification and Characterization of Bacterial Isolates from Spring Lake, Texas
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Eight bacterial isolates were retrieved from a biofilm during a homoserine lactone recruitment experiment in Spring Lake, Texas. On the basis of morphology, cellular fatty acid analysis, and 16S-23S intergenic transcribed spacer region sequencing, seven of the eight isolates were identified as Chromobacterium violaceum. A polyphasic approach to taxonomy was used to characterize the eighth, potentially novel isolate, A62-14B. The isolate was identified as a gamma proteobacterium, sharing 95-96% 16S rRNA gene sequence similarity to the genera Rheinheimera and Alishewanella. The 16S rRNA gene sequence data allowed for a novel species designation for isolate A62-14B, but did not resolve genus level relationships. Fatty acid analysis supported an association at the genus level of isolate A62-14B with Rheinheimera. Further physiological and morphological characterization via biochemical tests and electron microscopy revealed that the isolate shared a variety of key phenotypic traits with both related genera. It was concluded that isolate A62-14B represented a novel species, but a generic designation could not be assigned. The characterization of this organism revealed the short comings currently in bacterial taxonomy, including issues with a lack of taxon sampling, inability to compare and fully characterize unculturable organisms, and the lack of stringent control over experimental conditions for the comparison of organisms.
CitationMerchant, M. M. (2005). Identification and characterization of bacterial isolates from Spring Lake, Texas (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas.
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