The Distribution of Bone Sialoprotein in Bone Mineralization
Abstract
Bone consists of an organic matrix and hydroxyapatite (HA) crystals. The
mechanism by which bone mineralizes the organic matrix is not clearly understood.
Collagen provides the framework of bone and may even play a role in the nucleation of
the HA crystals through the hole zones within collagen fibers. The hole zones are created
by the alternating arrangement of the individual collagen fibrils. Phosphoproteins that
are only found in bone are thought to play an important role in the nucleation and
organization of the crystals. Bone sialoprotein (BSP) is a bone matrix protein that
possesses an Arg-Gly-Asp (RGD) cell attachment region and three polyglutamic acid
regions. These two regions of the protein are thought to have an active role in bone
mineralization. The RGD region has been shown to bind integrins on the surface of
osteoblasts. The negative charge of the glutamic acid regions can bind to HA crystals
and also may attract calcium ions that are incorporated into the HA crystals. BSP is
heavily phosphorylated through associations between BSP’s serine and threonine content
and inorganic phosphate ions. These phosphate ions may be available for use in the
mineralization process. It is thought that BSP is aiding in the nucleation of the crystals.
In the literature, there exist little histological characterizations of this protein in mature
bone. The organization of BSP within already ossified bone may help to ascertain its
function. Fish, chicken and pork bone were chosen for their differences in their
organization of mineralized bone and growth patterns. Immunohistological sections were
made of each bone type to try to identify the organization of the BSP in each. BSP was
found to be a component of the organic matrix. Moreover, BSP was more concentrated
in areas where there was active mineralization.
Citation
Plummer, C. T. (2002). The distribution of bone sialoprotein in bone mineralization (Unpublished thesis). Southwest Texas State University, San Marcos, Texas.Download
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