Effect of pokeweed antiviral protein on the induction of apoptosis in largemouth bass virus infected cells
Date
2006-06
Authors
Treadaway, Brent L.
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Abstract
Largemouth Bass Virus (LMBV), a member of the family Iridoviridae, infects warm freshwater fish, Asian ornamental fish, and is responsible for the killing of Largemouth Bass (Micropterus salmoides). This study demonstrates that ultraviolet lightinactivated LMBV induces apoptosis in Bluegill fry-2 (BF-2) infected cell monolayers via the extrinsic (caspase-8) pathway. Addition of pokeweed antiviral protein (PAP) to virus infected cells at a concentration sufficient to inhibit viral replication failed to inhibit LMBV-induced apoptosis. The results demonstrate LMBV-induced apoptosis is not dependent on viral protein synthesis and LMBV induced apoptosis occurs early in the infection cycle prior to host cell shut down and viral early gene expression. Apoptosis was observed 6h p.i. (post infection) following the addition of UV inactivated virus and 12h p.i. following the addition of infectious virus. The data suggest LMBV infection inhibits the extrinsic apoptotic pathway. Viral protein synthesis is necessary to re-initiate apoptosis via the intrinsic (caspase-9) pathway which occurs at 18h p.i. Activation of the caspase-9 pathway may be a response to viral induced cell damage.
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Keywords
Fishes, Pokeweed, Apoptosis, Largemouth bass
Citation
Treadaway, B. L. (2006). <i>Effect of pokeweed antiviral protein on the induction of apoptosis in largemouth bass virus infected cells</i> (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas.