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dc.contributor.authorSchartl, Manfred ( Orcid Icon 0000-0001-9882-5948 )
dc.contributor.authorShen, Yingjia ( )
dc.contributor.authorMaurus, Katja ( Orcid Icon 0000-0002-3093-3114 )
dc.contributor.authorWalter, Ronald B. ( )
dc.contributor.authorTomlinson, Chad ( )
dc.contributor.authorWilson, Richard K. ( )
dc.contributor.authorPostlethwait, John ( Orcid Icon 0000-0002-5476-2137 )
dc.contributor.authorWarren, Wesley C. ( )
dc.date.accessioned2021-08-10T14:43:03Z
dc.date.available2021-08-10T14:43:03Z
dc.date.issued2015-12-29
dc.identifier.citationSchartl, M., Shen, Y., Maurus, K., Walter, R., Tomlinson, C., Wilson, R. K., Postlethwait, J., & Warren, W. C. (2015). Whole body melanoma transcriptome response in medaka. PLoS One, 10(12), e0143057.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/14263
dc.description.abstractThe incidence of malignant melanoma continues to increase each year with poor prognosis for survival in many relapse cases. To reverse this trend, whole body response measures are needed to discover collaborative paths to primary and secondary malignancy. Several species of fish provide excellent melanoma models because fish and human melanocytes both appear in the epidermis, and fish and human pigment cell tumors share conserved gene expression signatures. For the first time, we have examined the whole body transcriptome response to invasive melanoma as a prelude to using transcriptome profiling to screen for drugs in a medaka (Oryzias latipes) model. We generated RNA-seq data from whole body RNA isolates for controls and melanoma fish. After testing for differential expression, 396 genes had significantly different expression (adjusted p-value <0.02) in the whole body transcriptome between melanoma and control fish; 379 of these genes were matched to human orthologs with 233 having annotated human gene symbols and 14 matched genes that contain putative deleterious variants in human melanoma at varying levels of recurrence. A detailed canonical pathway evaluation for significant enrichment showed the top scoring pathway to be antigen presentation but also included the expected melanocyte development and pigmentation signaling pathway. Results revealed a profound down-regulation of genes involved in the immune response, especially the innate immune system. We hypothesize that the developing melanoma actively suppresses the immune system responses of the body in reacting to the invasive malignancy, and that this mal-adaptive response contributes to disease progression, a result that suggests our whole-body transcriptomic approach merits further use. In these findings, we also observed novel genes not yet identified in human melanoma expression studies and uncovered known and new candidate drug targets for further testing in this malignant melanoma medaka model.en_US
dc.formatText
dc.format.extent14 pages
dc.format.medium1 file (.pdf)
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.sourcePLoS One, 2015, Vol. 10, No. 12, Article e0143057.
dc.subjectMelanomaen_US
dc.subjectGene expressionen_US
dc.subjectGene regulationen_US
dc.subjectMalignant tumorsen_US
dc.subjectRegulator genesen_US
dc.subjectMelanocytesen_US
dc.subjectCutaneous melanomaen_US
dc.subjectTranscriptome analysisen_US
dc.titleWhole Body Melanoma Transcriptome Response in Medakaen_US
dc.typepublishedVersion
txstate.documenttypeArticle
dc.rights.holder© 2015 Schartl et al.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0143057
dc.rights.licenseCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
dc.description.departmentChemistry and Biochemistry


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