Purification and Characterization of 2'-Hydrozybiphenyl-2-Sulfinate Desulfinase (DSZB) using the Substrate Analog Thiourea Dioxide
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The DszB enzyme, found in Rhodococcus erythropolis, is part of a metabolic desulfurization pathway which can be used to remove harmful sulfur from crude oil. The mechanism of this enzyme was studied using inhibition, inactivation, and alternative substrate studies. The possibility of DszB using a covalent mechanism was dismissed but either a non-covalent mechanism or a reversible covalent mechanism is still a viable option for this enzyme. Further studies are needed to better distinguish between the two current mechanism theories.