Show simple item record

dc.contributor.advisorRudzinski, Walter E.
dc.contributor.authorPalacios, Adriana S.
dc.date.accessioned2015-04-08T19:54:49Z
dc.date.available2015-04-08T19:54:49Z
dc.date.created2012-12
dc.date.issued2012-08-20
dc.date.submittedDecember 2012
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/5487
dc.description.abstractRNA interference (RNAi) is a recently discovered phenomenon that employs the use of miRNA (microRNA) and siRNA (short interfering RNA) that can be utilized for gene therapy purposes. However, the problem that has arisen is delivery of the siRNAs into diseased cells. The compound chitosan has recently gained much attention because it possesses several properties that make it an ideal candidate as a delivery vehicle for siRNA. Chitosan/siRNA based nanoparticles have been synthesized and characterized that are less than 100 nm in diameter. Characterization of their size was completed by using transmission electron microscopy (TEM), scanning electron microscopy (SEM), and dynamic light scattering (DLS) techniques. Characterization of siRNA loading was accomplished by utilizing UV spectroscopy, EDS, and confocal microscopy to test encapsulation of siRNA. The colorectal cancer cell line HCT 116, was used to test transfection of siRNA across the cellular membrane. The transfection efficiencies were evaluated by using fluorescently tagged siRNA embedded within chitosan nanoparticles. The chitosan/siRNA delivery system was compared with the classical approach used for siRNA delivery; namely, siRNA in Lipofectamine 2000 as well as with cells that received no treatment. Live cell imaging was also employed to aid in visualization of transfection. The results show that the chitosan based systems do transfect siRNA across the cellular member.
dc.formatText
dc.format.extent63 pages
dc.format.medium1 file (.pdf)
dc.format.medium2 files (.wmv)
dc.language.isoen_US
dc.subjectTransfection efficiency
dc.subjectsiRNA
dc.subjectChitosan nanoparticles
dc.titleTransfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells
txstate.documenttypeThesis
dc.contributor.committeeMemberWhitten, Steven T.
dc.contributor.committeeMemberBetancourt, Tania
thesis.degree.departmentChemistry and Biochemistry
thesis.degree.disciplineBiochemistry
thesis.degree.grantorTexas State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
txstate.departmentChemistry and Biochemistry


Download

Thumbnail

This item appears in the following Collection(s)

Show simple item record