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dc.contributor.advisorMcLean, Robert J. C.
dc.contributor.authorAltameemi, Marwa M. ( )
dc.date.accessioned2016-08-24T19:31:35Z
dc.date.available2016-08-24T19:31:35Z
dc.date.issued2013-08
dc.identifier.citationAltameemi, M. M. (2013). Methods development for exploring probiotic-host interactions in a C. elegans model (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas.
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/6276
dc.description.abstractCaenorhabditis elegans is a small round worm that can usually be found in the soil of temperate climates worldwide. It feeds on E. coli OP50; for the purpose of this study, we monitored these nematodes after they ingested probiotics, specifically B. longum, in a contamination free liquid media. The results show that when these worms consume B. longum, they stop growing at the L2 growth stage of the second generation, and they resume growing normally after adding E. coli OP50 into the media; perhaps, they required a specific metabolite chemicals provided in OP50. It has been further shown that they die when fed with L. casei as alternate food source to E. coli. The amounts of B. longum inside the worm’s gut shows that contained B. longum at numbers similar to human small intestine, with no OP50 detected.
dc.formatText
dc.format.extent85 pages
dc.format.medium1 file (.pdf)
dc.language.isoen
dc.subjectC. elegans
dc.subjectProbiotics
dc.subjectObesity
dc.subjectMicrobiota
dc.subjectB. longum
dc.subjectL. casei
dc.titleMethods Development for Exploring Probiotic-Host Interactions in a C. Elegans Model
txstate.documenttypeThesis
dc.contributor.committeeMemberMaitin, Vatsala
dc.contributor.committeeMemberVattem, Dhiraj
thesis.degree.departmentBiology
thesis.degree.disciplineBiology
thesis.degree.grantorTexas State University--San Marcos
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
txstate.departmentBiology


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