Fluorescent Light Incites a Conserved Immune and Inflammatory Genetic Response within Vertebrate Organs (Danio rerio, Oryzias latipes and Mus musculus)

Date

2019-04

Authors

Boswell, Mikki
Lu, Yuan
Savage, Markita G.
Hildreth, Kim
Salinas, Raquel
Walter, Christi A.
Walter, Ronald B.

Journal Title

Journal ISSN

Volume Title

Publisher

Multidisciplinary Digital Publishing Institute

Abstract

Fluorescent light (FL) has been utilized for ≈60 years and has become a common artificial light source under which animals, including humans, spend increasing amounts of time. Although the solar spectrum is quite dissimilar in both wavelengths and intensities, the genetic consequences of FL exposure have not been investigated. Herein, we present comparative RNA-Seq results that establish expression patterns within skin, brain, and liver for Danio rerio, Oryzias latipes, and the hairless mouse (Mus musculus) after exposure to FL. These animals represent diurnal and nocturnal lifestyles, and ≈450 million years of evolutionary divergence. In all three organisms, FL induced transcriptional changes of the acute phase response signaling pathway and modulated inflammation and innate immune responses. Our pathway and gene clustering analyses suggest cellular perception of oxidative stress is promoting induction of primary up-stream regulators IL1B and TNF. The skin and brain of the three animals as well as the liver of both fish models all exhibit increased inflammation and immune responses; however, the mouse liver suppressed the same pathways. Overall, the conserved nature of the genetic responses observed after FL exposure, among fishes and a mammal, suggest the presence of light responsive genetic circuitry deeply embedded in the vertebrate genome.

Description

Keywords

RNA-Seq, fluorescent light, acute phase, oxidative stress, gene expression, Chemistry and Biochemistry

Citation

Boswell, M., Lu, Y., Boswell, W., Savage, M., Hildreth, K., Salinas, R., Walter, C. A., & Walter, R. B. (2019). Fluorescent Light Incites a Conserved Immune and Inflammatory Genetic Response within Vertebrate Organs (Danio rerio, Oryzias latipes and Mus musculus). Genes, 10(4): 271.

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© 2019 The Authors.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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