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dc.contributor.authorNuckels, Richard J.
dc.contributor.authorNice, Chris C.
dc.contributor.authorGarcia, Dana M.
dc.date.accessioned2019-08-05T16:47:44Z
dc.date.available2019-08-05T16:47:44Z
dc.date.issued2019-11-29
dc.identifier.citationNuckels, R. J., Nice, C. C., & García, D. M. (2019). Duplicated Myosin V Genes in Teleosts Show Evolutionary Rate Variations among the Motor and Cargo-Binding Domains. Genome Biology And Evolution, 11(2), pp. 415–430.en_US
dc.identifier.issn1759-6653
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/8452
dc.description.abstractWe analyzed evolutionary rates of conserved, duplicated myosin V (myo5) genes in nine teleost species to examine the outcomes of duplication events. Syntenic analysis and ancestral chromosome mapping suggest one tandem gene duplication event leading to the appearance of myo5a and myo5c, two rounds of whole genome duplication for vertebrates, and an additional round of whole genome duplication for teleosts account for the presence and location of the myo5 genes and their duplicates in teleosts and other vertebrates and the timing of the duplication events. Phylogenetic analyses reveal a previously unidentified myo5 clade that we refer to now as myo5bb. Analysis using dN/dS rate comparisons revealed large regions within duplicated myo5 genes that are highly conserved. Codons identified in other studies as encoding functionally important portions of the Myo5a and Myo5b proteins are shown to be highly conserved within the newly identified myo5bb clade and in other myo5 duplicates. As much as 30% of 319 codons encoding the cargo-binding domain in the myo5aa genes are conserved in all three codon positions in nine teleost species. For the myo5bb cargo-binding domain, 6.6% of 336 codons have zero substitutions in all nine teleost species. Using molecular evolution assays, we identify the myo5bb branch as being subject to evolutionary rate variation with the cargo-binding domain, having 20% of the sites under positive selection and the motor domain having 8% of its sites under positive selection. The high number of invariant codons coupled with relatively high dN/dS values in the region of the myo5 genes encoding the ATP-binding domain suggests the encoded proteins retain function and may have acquired novel functions associated with changes to the cargo-binding domain.en_US
dc.formatText
dc.format.extent16 pages
dc.format.medium1 file (.pdf)
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.sourceGenome Biology And Evolution, 2019, Vol. 11, No. 2, pp. 415–430
dc.subjectGene duplicationen_US
dc.subjectMyosin V
dc.subjectCargo-binding domain
dc.subjectMotor domain
dc.subjectInvariant codon
dc.subjectdN/dS
dc.subjectEvolutionary rates
dc.titleDuplicated Myosin V Genes in Teleosts Show Evolutionary Rate Variations among the Motor and Cargo-Binding Domainsen_US
txstate.documenttypeArticle
dc.identifier.doihttps://doi.org/10.1093/gbe/evy258
dc.rights.licenseThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
txstate.departmentBiology


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