Irreversible Protein Labeling by Paal-Knorr Conjugation

Date

2017-09

Authors

Dasari, Ramesh
La Clair, James
Kornienko, Alexander

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Publisher

Wiley

Abstract

The application of new chemical reactions in a biological context has advanced bioconjugation methods for both fundamental research and commercial arenas. Recent adaptations of reactions such as Huisgen 1,3-dipolar or Diels-Alder cycloadditions have enabled the labeling of specific residues in biomolecules by the attachment of molecules carrying azides, alkynes, or strained alkenes. Although these are fundamental tools, there is a need for the discovery of reactions that can label native proteins. We report herein the adaptation of the Paal-Knorr reaction to label lysine residues in proteins via pyrrole linkages.

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Keywords

biocompatible reactions, bioconjugation, click chemistry, protein labeling, Paal-Knorr reaction, Chemistry and Biochemistry

Citation

Dasari, R., La Clair, J. J., & Kornienko, A. (2017). Irreversible protein labeling by Paal-Knorr conjugation. ChemBioChem, 18(18), pp. 1792–1796.

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