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dc.contributor.authorZhao, Zhenze ( )
dc.contributor.authorMa, Xiuye ( )
dc.contributor.authorShelton, Spencer D. ( )
dc.contributor.authorSung, Derek C. ( )
dc.contributor.authorLi, Monica ( )
dc.contributor.authorHernandez, Daniel ( )
dc.contributor.authorZhang, Maggie ( )
dc.contributor.authorLosiewicz, Michael D. ( )
dc.contributor.authorChen, Yidong ( )
dc.contributor.authorPertsemlidis, Alexander ( Orcid Icon 0000-0003-1624-9372 )
dc.contributor.authorYu, Xiaojie ( Orcid Icon 0000-0001-5586-0428 )
dc.contributor.authorLiu, Yuanhang ( )
dc.contributor.authorDu, Liqin ( )
dc.date.accessioned2020-04-15T19:41:44Z
dc.date.available2020-04-15T19:41:44Z
dc.date.issued2016-10
dc.identifier.citationZhao, Z., Ma, X., Shelton, S. D., Sung, D. C., Li, M., Hernandez, D., Zhang, M., Losiewicz, M. D., Chen, Y., Pertsemlidis, A., Yu, X., Liu, Y., & Du, L. (2016). A combined gene expression and functional study reveals the crosstalk between N-Myc and differentiation-inducing microRNAs in neuroblastoma cells. Oncotarget, 7(48), pp. 79358–79373.en_US
dc.identifier.issn1949-2553
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/9619
dc.description.abstractMYCN amplification is the most common genetic alteration in neuroblastoma and plays a critical role in neuroblastoma tumorigenesis. MYCN regulates neuroblastoma cell differentiation, which is one of the mechanisms underlying its oncogenic function. We recently identified a group of differentiation-inducing microRNAs. Given the demonstrated inter-regulation between MYCN and microRNAs, we speculated that MYCN and the differentiation-inducing microRNAs might form an interaction network to control the differentiation of neuroblastoma cells. In this study, we found that eight of the thirteen differentiation-inducing microRNAs, miR-506-3p, miR-124-3p, miR-449a, miR-34a-5p, miR-449b-5p, miR-103a-3p, miR-2110 and miR-34b-5p, inhibit N-Myc expression by either directly targeting the MYCN 3'UTR or through indirect regulations. Further investigation showed that both MYCN-dependent and MYCN-independent pathways play roles in mediating the differentiation-inducing function of miR-506-3p and miR-449a, two microRNAs that dramatically down-regulate MYCN expression. On the other hand, we found that N-Myc inhibits the expression of multiple differentiation-inducing microRNAs, suggesting that these miRNAs play a role in mediating the function of MYCN. In examining the published dataset collected from clinical neuroblastoma specimens, we found that expressions of two miRNAs, miR-137 and miR-2110, were significantly anti-correlated with MYCN mRNA levels, suggesting their interactions with MYCN play a clinically-relevant role in maintaining the MYCN and miRNA expression levels in neuroblastoma. Our findings altogether suggest that MYCN and differentiation-inducing miRNAs form an interaction network that play an important role in neuroblastoma tumorigenesis through regulating cell differentiation.en_US
dc.formatText
dc.format.extent16 pages
dc.format.medium1 file (.pdf)
dc.language.isoen
dc.publisherImpact Journalsen_US
dc.sourceOncotarget, 2016, Vol. 7, No. 48, pp. 79358–79373.
dc.subjectMYCNen_US
dc.subjectDifferentiation
dc.subjectmicroRNA
dc.subjectNeuroblastoma
dc.titleA Combined Gene Expression and Functional Study Reveals the Crosstalk Between N-Myc and Differentiation-inducing MicroRNAs in Neuroblastoma Cellsen_US
txstate.documenttypeArticle
dc.identifier.doihttps://doi.org/10.18632/oncotarget.12676
txstate.departmentChemistry and Biochemistry


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