Synthesis and Evaluation of a Rationally Designed Click-Based Library for G-Quadruplex Selective DNA Photocleavage
Date
2015-09
Authors
McBrayer, Dominic
Kerwin, Sean M.
Journal Title
Journal ISSN
Volume Title
Publisher
Multidisciplinary Digital Publishing Institute
Abstract
DNA containing repeating G-rich sequences can adopt higher-order structures known as G-quadruplexes (G4). These structures are believed to form within telomeres and the promoter regions of some genes, particularly in a number of proto-oncogenes, where they may play a role in regulating transcription. Alternatively, G4 DNA may act as a barrier to replication. To investigate these potential biological roles, probes that combine highly selective G4 DNA targeting with photocleavage activity can allow temporal detection of G4 DNA, providing opportunities to obtain novel insights about the biological roles of G4 DNA. We have designed, synthesized, and screened a small library of potential selective G-quadruplex DNA photocleavage agents incorporating the G-quadruplex targeting moiety of 360A with known photocleavage groups linked via “click” chemistry.
Description
Keywords
DNA, Photocleavage, Alkyne-azide click reaction, PAGE
Citation
McBrayer, D., & Kerwin, S. M. (2015). Synthesis and evaluation of a rationally designed click-based library for G-quadruplex selective DNA photocleavage. Molecules, 20(9), pp. 16446-16465.
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This work is licensed under a Creative Commons Attribution 4.0 International License.