microRNA-2110 Functions as an Onco-suppressor in Neuroblastoma by Directly Targeting Tsukushi

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dc.contributor.authorZhao, Zhenze
dc.contributor.authorPartridge, Veronica
dc.contributor.authorSousares, Michaela
dc.contributor.authorShelton, Spencer D.
dc.contributor.authorHolland, Cory L.
dc.contributor.authorPertsemlidis, Alexander
dc.contributor.authorDu, Liqin
dc.date.accessioned2019-08-02T18:10:55Z
dc.date.available2019-08-02T18:10:55Z
dc.date.issued2018-12-14
dc.description.abstractmicroRNA-2110 (miR-2110) was previously identified as inducing neurite outgrowth in a neuroblastoma cell lines BE(2)-C, suggesting its differentiation-inducing and oncosuppressive function in neuroblastoma. In this study, we demonstrated that synthetic miR-2110 mimic had a generic effect on reducing cell survival in neuroblastoma cell lines with distinct genetic backgrounds, although the induction of cell differentiation traits varied between cell lines. In investigating the mechanisms underlying such functions of miR-2110, we identified that among its predicted target genes down-regulated by miR-2110, knockdown of Tsukushi (TSKU) expression showed the most potent effect in inducing cell differentiation and reducing cell survival, suggesting that TSKU protein plays a key role in mediating the functions of miR-2110. In investigating the clinical relevance of miR-2110 and TSKU expression in neuroblastoma patients, we found that low tumor miR-2110 levels were significantly correlated with high tumor TSKU mRNA levels, and that both low miR-2110 and high TSKU mRNA levels were significantly correlated with poor patient survival. These findings altogether support the oncosuppressive function of miR-2110 and suggest an important role for miR-2110 and its target TSKU in neuroblastoma tumorigenesis and in determining patient prognosis.
dc.description.departmentChemistry and Biochemistry
dc.formatText
dc.format.extent19 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationZhao, Z., Partridge, V., Sousares, M., Shelton, S. D., Holland, C. L., Pertsemlidis, A., Du, L. (2018) microRNA-2110 functions as an onco-suppressor in neuroblastoma by directly targeting Tsukushi. PLoS ONE, 13(12) : e0208777.
dc.identifier.doihttps://doi.org/ 10.1371/journal.pone.0208777
dc.identifier.urihttps://hdl.handle.net/10877/8448
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holder© 2018 Zhao et al.
dc.rights.licenseThis work is licensed under a Creative Commons Attribution 4.0 International License.
dc.sourcePLoS ONE, 2018, Vol. 13, No. 12 : e0208777.
dc.subjectmicroRNA-2110
dc.subjectneuroblastoma cell lines
dc.subjectoncosuppressive function
dc.subjectTsukushi (TSKU)
dc.subjectChemistry and Biochemistry
dc.titlemicroRNA-2110 Functions as an Onco-suppressor in Neuroblastoma by Directly Targeting Tsukushi
dc.typeArticle

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