Real-Time Interactions Between SV40 T-Antigen and a Stream of Analytes
dc.contributor.author | David, Wendi M. | |
dc.date.accessioned | 2009-08-11T10:06:37Z | |
dc.date.available | 2012-02-24T10:07:01Z | |
dc.date.issued | 2008-01 | |
dc.description | Research Enhancement Program Final Report | |
dc.description.abstract | The primary goal of the funded research involved using surface plasmon resonance (SPR) for determining real-time interactions between viral SV40 large T-antigen (T-ag), DNA, and a stream of quadruplex DNA-interactive small molecules. We successfully developed an SPR-based assay for monitoring the ability of T-ag to unwind both normal duplex DNA and quadruplex DNA and characterized the ability of several small molecules to inhibit the helicase activity of T-ag. The ability to monitor unwinding of quadruplex DNA in real-time is an advance for investigating the inhibitory effects of quadruplex-interactive agents and this work was published recently in Cell Biochemistry and Biophysics (Feb. 2009). Our results will aid in future characterization of potential therapeutic approaches involving G-quadruplex interactive small molecules and in understanding the structural basis for selective targeting of different families of G-quadruplex DNA helicases. These results form the basis for a recent NIH ARRA grant in collaboration with UT-Austin. In addition, data generated in this research was presented at poster presentations for three national biochemistry meetings. A total of 4 undergraduate students and 1 graduate student were involved in the research. | |
dc.description.department | Sponsored Programs | |
dc.format | Text | |
dc.format.extent | 1 page | |
dc.format.medium | 1 file (.pdf) | |
dc.identifier.citation | David, W. M. (2008). Real-time interactions between SV40 T-antigen and a stream of analytes. Research Enhancement Program, Texas State University, San Marcos, Texas. | |
dc.identifier.uri | https://hdl.handle.net/10877/2899 | |
dc.language.iso | en | |
dc.subject | viral SV40 | |
dc.subject | T-antigen | |
dc.subject | T-ag | |
dc.subject | DNA | |
dc.subject | analytes | |
dc.subject | plasmon resonance | |
dc.subject | SPR | |
dc.title | Real-Time Interactions Between SV40 T-Antigen and a Stream of Analytes | |
dc.type | Report |
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