Enhancing Metagenomics Investigations of Microbial Interactions with Biofilm Technology

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dc.contributor.authorMcLean, Robert J. C.
dc.contributor.authorKakirde, Kavita S.
dc.date.accessioned2021-06-29T20:08:46Z
dc.date.available2021-06-29T20:08:46Z
dc.date.issued2013-11-11
dc.description.abstractInvestigations of microbial ecology and diversity have been greatly enhanced by the application of culture-independent techniques. One such approach, metagenomics, involves sample collections from soil, water, and other environments. Extracted nucleic acids from bulk environmental samples are sequenced and analyzed, which allows microbial interactions to be inferred on the basis of bioinformatics calculations. In most environments, microbial interactions occur predominately in surface-adherent, biofilm communities. In this review, we address metagenomics sampling and biofilm biology, and propose an experimental strategy whereby the resolving power of metagenomics can be enhanced by incorporating a biofilm-enrichment step during sample acquisition.
dc.description.departmentBiology
dc.formatText
dc.format.extent12 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationMcLean, R. J. C., & Kakirde, K. (2013). Enhancing metagenomics investigations of microbial interactions with biofilm technology. International Journal of Molecular Sciences, 14(11), pp. 22246-22257.
dc.identifier.doihttps://doi.org/10.3390/ijms141122246
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/10877/13794
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute
dc.rights.holder© 2013 The Authors.
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
dc.sourceInternational Journal of Molecular Sciences, Vol. 14, No. 11, pp. 22246-22257.
dc.subjectbiofilm-enrichment
dc.subjectmetagenomics
dc.subjectfunctional screening
dc.subjectsequence-based screening
dc.subjectmicrobial interactions
dc.subjectBiology
dc.titleEnhancing Metagenomics Investigations of Microbial Interactions with Biofilm Technology
dc.typeArticle

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