Taxonomic and Metabolic Incongruence in the Ancient Genus Streptomyces

Simple item page
dc.contributor.authorChevrette, Marc G.
dc.contributor.authorCarlos-Shanley, Camila
dc.contributor.authorLouie, Katherine B.
dc.contributor.authorBowen, Benjamin
dc.contributor.authorNorthen, Trent R.
dc.contributor.authorCurrie, Cameron R.
dc.date.accessioned2019-10-18T19:30:18Z
dc.date.available2019-10-18T19:30:18Z
dc.date.issued2019-09
dc.description.abstractThe advent of culture independent approaches has greatly facilitated insights into the vast diversity of bacteria and the ecological importance they hold in nature and human health. Recently, metagenomic surveys and other culture-independent methods have begun to describe the distribution and diversity of microbial metabolism across environmental conditions, often using 16S rRNA gene as a marker to group bacteria into taxonomic units. However, the extent to which similarity at the conserved ribosomal 16S gene correlates with different measures of phylogeny, metabolic diversity, and ecologically relevant gene content remains contentious. Here, we examine the relationship between 16S identity, core genome divergence, and metabolic gene content across the ancient and ecologically important genus Streptomyces. We assessed and quantified the high variability of average nucleotide identity (ANI) and ortholog presence/absence within Streptomyces, even in strains identical by 16S. Furthermore, we identified key differences in shared ecologically important characters, such as antibiotic resistance, carbohydrate metabolism, biosynthetic gene clusters (BGCs), and other metabolic hallmarks, within 16S identities commonly treated as the same operational taxonomic units (OTUs). Differences between common phylogenetic measures and metabolite-gene annotations confirmed this incongruence. Our results highlight the metabolic diversity and variability within OTUs and add to the growing body of work suggesting 16S-based studies of Streptomyces fail to resolve important ecological and metabolic characteristics.
dc.description.departmentBiology
dc.formatText
dc.format.extent12 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationChevrette, M. G., Carlos-Shanley, C., Louie, K. B., Bowen, B. P., Northen, T. R., & Currie, C. R. (2019). Taxonomic and metabolic incongruence in the Ancient genus streptomyces. <i>Frontiers in Microbiology, 10</i> : 2170.
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.02170
dc.identifier.issn1664-302X
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/10877/8719
dc.language.isoen
dc.publisherFrontiers Media
dc.rights.holder© 2019 Chevrette, Carlos-Shanley, Louie, Bowen, Northen and Currie.
dc.rights.licenseThis work is licensed under a Creative Commons Attribution 4.0 International License.
dc.sourceFrontiers in Microbiology, 2019, Vol. 10, Article 2170
dc.subjectmetabolism
dc.subject16S
dc.subjectphylogenomics
dc.subjectmetabolites
dc.subjectstreptomyces
dc.subjectBiology
dc.titleTaxonomic and Metabolic Incongruence in the Ancient Genus Streptomyces
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
fmicb-10-02170.pdf
Size:
12.1 MB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
2.54 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
No Thumbnail Available
Name:
PERMISSION-Frontiers-Microbiology.pdf
Size:
157.15 KB
Format:
Adobe Portable Document Format
Description:
Download

Collections

College of Science and Engineering