Purification of the alpha subunit of the epithelial sodium channel (αENaC) for Surface Plasmon Resonance (SPR) studies

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dc.contributor.advisorBooth, Rachell
dc.contributor.authorBerman, Chance N.
dc.contributor.committeeMemberDavid, Wendi
dc.contributor.committeeMemberLewis, Karen
dc.date.accessioned2017-01-06T17:53:33Z
dc.date.available2017-01-06T17:53:33Z
dc.date.issued2016-12
dc.description.abstractThe epithelial sodium channel (ENaC) is responsible for sodium reabsorption in the distal convoluted tubules of the nephron in the kidneys. ENaC is a transmembrane protein with N and C-termini located in the cytosol and a larger extracellular loop containing a wrist, palm, beta-ball, thumb, knuckle and finger. Due to ENaCs hydrophilic and hydrophobic nature, isolation and purification while still being functional is not well understood. A 6x histidine tag was engineered into the extracellular loop of αENaC, referred to hereon as αHis2-αENaC, and then was subcloned in the yeast expression vector pYES2/NTA. pYES2/NTA/αHis2-αENaC was transformed into S1, MATα ura3-52 leu2-3,112 trp1-289 his7-2 ade5-1 lys2::InsE-4A, and BY4742, MATα his3∆1 leu2∆ lys2∆ ura3∆ ygr204::kanMX4, yeast strains. Serial dilution assays, time-course expression trials, immobilized metal affinity chromatography (IMAC) and western blot analysis were employed to purify and verify expression of αHis2 in each strain of Saccharomyces cerevisae. ENaC peptides of each subunit were previously engineered and subcloned in the bacterial expression vector pGEX-4T-2. Immobilized substrate affinity chromatography (ISAC) was employed to purify peptides. Surface Plasmon Resonance (SPR) was conducted between ENaC peptides and whole subunit αHis2-αENaC. We report that the interactions of the palm and thumb region of βENaC, independently, between the whole αENaC subunit gave kinetic constants in the non-covalent range, potentially being an inter-subunit binding domain. Additional studies would use longer peptides, thought to be portions/ whole binding domains, to further elucidate inter- subunit binding regions.
dc.description.departmentChemistry and Biochemistry
dc.formatText
dc.format.extent86 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationBerman, C. N. (2016). <i>Purification of the alpha subunit of the epithelial sodium channel (αENaC) for Surface Plasmon Resonance (SPR) studies</i> (Unpublished thesis). Texas State University, San Marcos, Texas.
dc.identifier.urihttps://hdl.handle.net/10877/6408
dc.language.isoen
dc.subjectEpithelial sodium channel
dc.subjectSurface Plasmon Resonance
dc.subject.lcshSodium channelsen_US
dc.subject.lcshEpitheliumen_US
dc.subject.lcshSurface plasmon resonanceen_US
dc.titlePurification of the alpha subunit of the epithelial sodium channel (αENaC) for Surface Plasmon Resonance (SPR) studies
dc.typeThesis
thesis.degree.departmentChemistry and Biochemistry
thesis.degree.disciplineBiochemistry
thesis.degree.grantorTexas State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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