Nano-bio-chip sensor platform for examination of oral exfoliative cytology

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dc.contributor.authorWeigum, Shannon E.
dc.contributor.authorFloriano, Pierre N.
dc.contributor.authorRedding, Spencer W.
dc.contributor.authorYeh, Chih-Ko
dc.contributor.authorWestbrook, Stephen D.
dc.contributor.authorMcGuff, H. Stan
dc.contributor.authorLin, Alan
dc.contributor.authorMiller, Frank R.
dc.contributor.authorVillarreal, Fred
dc.contributor.authorRowan, Stephanie D.
dc.contributor.authorVigneswaran, Nadarajah
dc.contributor.authorWilliams, Michelle D.
dc.contributor.authorMcDevitt, John T.
dc.date.accessioned2021-04-09T14:04:57Z
dc.date.available2021-04-09T14:04:57Z
dc.date.issued2010-04
dc.description.abstractOral cancer is a deadly and disfiguring disease that could greatly benefit from new diagnostic approaches enabling early detection. In this pilot study, we describe a nano-bio-chip (NBC) sensor technique for analysis of oral cancer biomarkers in exfoliative cytology specimens, targeting both biochemical and morphologic changes associated with early oral tumorigenesis. Here, oral lesions from 41 dental patients, along with normal epithelium from 11 healthy volunteers, were sampled using a noninvasive brush biopsy technique. Specimens were enriched, immunolabeled, and imaged in the NBC sensor according to previously established assays for the epidermal growth factor receptor (EGFR) biomarker and cytomorphometry. A total of 51 measurement parameters were extracted using custom image analysis macros, including EGFR labeling intensity, cell and nuclear size, and the nuclear-to-cytoplasmic ratio. Four key parameters were significantly elevated in both dysplastic and malignant lesions relative to healthy oral epithelium, including the nuclear area and diameter (P < 0.0001), the nuclear-to-cytoplasmic ratio (P < 0.0001), and EGFR biomarker expression (P < 0.03). Further examination using logistic regression and receiver operating characteristic curve analyses identified morphologic features as the best predictors of disease (area under the curve ≤0.93) individually, whereas a combination of all features further enhanced discrimination of oral cancer and precancerous conditions (area under the curve, 0.94) with high sensitivity and specificity. Further clinical trials are necessary to validate the regression model and evaluate other potential biomarkers, but this pilot study supports the NBC sensor technique as a promising new diagnostic tool for early detection of oral cancer, which could enhance patient care and survival.
dc.description.departmentBiology
dc.description.versionThis is the author accepted manuscript version of an article published in Cancer Prevention Research.
dc.formatText
dc.format.extent19 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationWeigum, S. E., Floriano, P. N., Redding, S. W., Yeh, C. K., Westbrook, S. D., McGuff, H. S., Lin, A., Miller, F. R., Villarreal, F., Rowan, S. D., Vigneswaran, N., Williams, M. D., McDevitt, J. T. (2010). Nano-bio-chip sensor platform for examination of oral exfoliative cytology. Cancer Prevention Research, 3(4), pp. 518-528.
dc.identifier.doihttps://doi.org/10.1158/1940-6207.CAPR-09-0139
dc.identifier.urihttps://hdl.handle.net/10877/13349
dc.language.isoen
dc.publisherAmerican Association for Cancer Research
dc.sourceCancer Prevention Research, 2010, Vo. 3, No. 4) pp. 518-528.
dc.subjectlab-on-a-chip sensor
dc.subjectoral cancer
dc.subjectbiomarkers
dc.subjectEGFR
dc.subjectexfoliative cytology
dc.subjectBiology
dc.titleNano-bio-chip sensor platform for examination of oral exfoliative cytology
dc.typeArticle

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