Effects of Aged Garlic Extract and FruArg on Gene Expression and Signaling Pathways in Lipopolysaccharide-activated Microglial Cells

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dc.contributor.authorSong, Hailong
dc.contributor.authorLu, Yuan
dc.contributor.authorQu, Zhe
dc.contributor.authorMossine, Valeri
dc.contributor.authorMartin, Matthew B.
dc.contributor.authorHou, Jie
dc.contributor.authorCui, Jiankun
dc.contributor.authorPeculis, Brenda A.
dc.contributor.authorMawhinney, Thomas
dc.contributor.authorCheng, Jianlin
dc.contributor.authorGreenlief, C. Michael
dc.contributor.authorFritsche, Kevin
dc.contributor.authorSchmidt, Francis J.
dc.contributor.authorWalter, Ronald B.
dc.contributor.authorLubahn, Dennis
dc.contributor.authorSun, Grace Y.
dc.contributor.authorGu, Zezong
dc.date.accessioned2020-04-16T15:22:25Z
dc.date.available2020-04-16T15:22:25Z
dc.date.issued2016-10
dc.description.abstractAged garlic extract (AGE) is widely used as a dietary supplement on account of its protective effects against oxidative stress and inflammation. But less is known about specific molecular targets of AGE and its bioactive components, including N-α-(1-deoxy-D-fructos-1-yl)-L-arginine (FruArg). Our recent study showed that both AGE and FruArg significantly attenuate lipopolysaccharide (LPS)-induced neuroinflammatory responses in BV-2 microglial cells. This study aims to unveil effects of AGE and FruArg on gene expression regulation in LPS stimulated BV-2 cells. Results showed that LPS treatment significantly altered mRNA levels from 2563 genes. AGE reversed 67% of the transcriptome alteration induced by LPS, whereas FruArg accounted for the protective effect by reversing expression levels of 55% of genes altered by LPS. Key pro-inflammatory canonical pathways induced by the LPS stimulation included toll-like receptor signaling, IL-6 signaling, and Nrf2-mediated oxidative stress pathway, along with elevated expression levels of genes, such as Il6, Cd14, Casp3, Nfkb1, Hmox1, and Tnf. These effects could be modulated by treatment with both AGE and FruArg. These findings suggests that AGE and FruArg are capable of alleviating oxidative stress and neuroinflammatory responses stimulated by LPS in BV-2 cells.
dc.description.departmentChemistry and Biochemistry
dc.formatText
dc.format.extent10 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationSong, H., Lu, Y., Qu, Z., Mossine, V. V., Martin, M. B., Hou, J., Cui, J., Peculis, B. A., Mawhinney, T. P., Cheng, J., Greenlief, C. M., Fritsche, K., Schmidt, F. J., Walter, R. B., Lubahn, D. B., Sun, G. Y., & Gu, Z. (2016). Effects of aged garlic extract and FruArg on gene expression and signaling pathways in lipopolysaccharide-activated microglial cells. Scientific Reports, 6: 35323.
dc.identifier.doihttps://doi.org/10.1038/srep35323
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10877/9620
dc.language.isoen
dc.publisherNature Research
dc.rights.holder© 2016 The Author(s).
dc.rights.licenseThis work is licensed under a Creative Commons Attribution 4.0 International License.
dc.sourceScientific Reports, 2016, Vol. 6, Article 35323.
dc.subjectaged garlic extract
dc.subjectFruArg
dc.subjectgene expression
dc.subjectsignaling pathways
dc.subjectlipopolysaccharide-activated
dc.subjectmicroglial cells
dc.subjectChemistry and Biochemistry
dc.titleEffects of Aged Garlic Extract and FruArg on Gene Expression and Signaling Pathways in Lipopolysaccharide-activated Microglial Cells
dc.typeArticle

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