Impact of Size, Secondary Structure, and Counterions on the Binding of Small Ribonucleic Acids to Layered Double Hydroxide Nanoparticles

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dc.contributor.authorRodriguez, Blanca V.
dc.contributor.authorPescador, Jorge
dc.contributor.authorPollok, Nicole
dc.contributor.authorBeall, Gary W.
dc.contributor.authorMaeder, Corina
dc.contributor.authorLewis, L. Kevin
dc.date.accessioned2020-05-26T15:28:58Z
dc.date.available2020-05-26T15:28:58Z
dc.date.issued2015-11
dc.description.abstractUse of ribonucleic acid (RNA) interference to regulate protein expression has become an important research topic and gene therapy tool, and therefore, finding suitable vehicles for delivery of small RNAs into cells is of crucial importance. Layered double metal hydroxides such as hydrotalcite (HT) have shown great promise as nonviral vectors for transport of deoxyribose nucleic acid (DNA), proteins, and drugs into cells, but the adsorption of RNAs to these materials has been little explored. In this study, the binding of small RNAs with different lengths and levels of secondary structure to HT nanoparticles has been analyzed and compared to results obtained with small DNAs in concurrent experiments. Initial experiments established the spectrophotometric properties of HT in aqueous solutions and determined that HT particles could be readily sedimented with near 100% efficiencies. Use of RNA+HT cosedimentation experiments as well as electrophoretic mobility shift assays demonstrated strong adsorption of RNA 25mers to HT, with twofold greater binding of single-stranded RNAs relative to double-stranded molecules. Strong affinities were also observed with ssRNA and dsRNA 54mers and with more complex transfer RNA molecules. Competition binding and RNA displacement experiments indicated that RNA-HT associations were strong and were only modestly affected by the presence of high concentrations of inorganic anions.
dc.description.departmentChemistry and Biochemistry
dc.formatText
dc.format.extent10 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationRodriguez, B. V., Pescador, J., Pollok, N., Beall, G. W., Maeder, C., & Lewis, L. K. (2015). Impact of size, secondary structure, and counterions on the binding of small ribonucleic acids to layered double hydroxide nanoparticles. Biointerphases, 10(4).
dc.identifier.doihttp://dx.doi.org/10.1116/1.4936393
dc.identifier.issn1934-8630
dc.identifier.urihttps://hdl.handle.net/10877/10310
dc.language.isoen
dc.publisherAmerican Vacuum Society
dc.rights.holder© 2015 American Vacuum Society.
dc.sourceBiointerphases, 2015, Vol. 10, No. 4, Article 041007.
dc.subjectribonucleic acid
dc.subjectnanoparticles
dc.subjectlayered double hydroxides
dc.subjectChemistry and Biochemistry
dc.titleImpact of Size, Secondary Structure, and Counterions on the Binding of Small Ribonucleic Acids to Layered Double Hydroxide Nanoparticles
dc.typeArticle

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