Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp
dc.contributor.author | Zhang, Bao | |
dc.contributor.author | Zhuang, Xiyi | |
dc.contributor.author | Guo, Liyun | |
dc.contributor.author | McLean, Robert J. C. | |
dc.contributor.author | Chu, Weihua | |
dc.date.accessioned | 2019-09-06T13:20:01Z | |
dc.date.available | 2019-09-06T13:20:01Z | |
dc.date.issued | 2019-08 | |
dc.description.abstract | Quorum quenching (QQ) is a promising alternative infection-control strategy to antibiotics that controls quorum-regulated virulence without killing the pathogens. Aeromonas hydrophila is an opportunistic gram-negative pathogen living in freshwater and marine environments. A. hydrophila possesses an N-acyl homoserine lactone (AHL)-based quorum-sensing (QS) system that regulates virulence, so quorum signal-inactivation (i.e., QQ) may represent a new way to combat A. hydrophila infection. In this study, an AHL lactonase gene, aiiA was cloned from Bacillus sp. strain QSI-1 and expressed in Escherichia coli strain BL21(DE3). The A. hydrophila hexanoyl homoserine lactone (C6-HSL) QS signal molecule was degraded by Aii AQSI-1, which resulted in a decrease of bacterial swimming motility, reduction of extracellular protease and hemolysin virulence factors, and inhibited the biofilm formation of A. hydrophila YJ-1 in a microtiter assay. In cell culture studies, Aii AQSI-1 decreased the ability of A. hydrophila adherence to and internalization by Epithelioma papulosum cyprini (EPC) cells. During in vivo studies, oral administration of AiiA QSI-1 via feed supplementation attenuated A. hydrophila infection in Crucian Carp. Results from this work indicate that feed supplementation with Aii AQSI-1 protein has potential to control A. hydrophila aquaculture disease via QQ. | |
dc.description.department | Biology | |
dc.format | Text | |
dc.format.extent | 12 pages | |
dc.format.medium | 1 file (.pdf) | |
dc.identifier.citation | Zhang, B., Zhuang, X., Guo, L., McLean, R. J. C., & Chu, W. (2019). Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp. Marine Drugs, 17(9): 499. | |
dc.identifier.doi | https://doi.org/10.3390/md17090499 | |
dc.identifier.uri | https://hdl.handle.net/10877/8603 | |
dc.language.iso | en | |
dc.publisher | Multidisciplinary Digital Publishing Institute | |
dc.rights.holder | © 2019 The Authors. | |
dc.rights.license | This work is licensed under a Creative Commons Attribution 4.0 International License. | |
dc.source | Marine Drugs, 2019, Vol. 17, No. 9, Article 499. | |
dc.subject | crucian Carp | |
dc.subject | N-acyl homoserine lactone lactonase | |
dc.subject | biofilm | |
dc.subject | virulence factors | |
dc.subject | aeromonas hydrophila | |
dc.subject | Biology | |
dc.title | Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp | |
dc.type | Article |