Molecular and Pharmacological Characterization of Muscarinic Receptors in Retinal Pigment Epithelium: Role in Light-adaptive Pigment Movements

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dc.contributor.authorPhatarpekar, Prasad V.
dc.contributor.authorDurdan, Simon F.
dc.contributor.authorCopeland, Chad M.
dc.contributor.authorCrittenden, Elizabeth L.
dc.contributor.authorNeece, James D.
dc.contributor.authorGarcia, Dana M.
dc.date.accessioned2016-11-09T20:10:44Z
dc.date.available2016-11-09T20:10:44Z
dc.date.issued2005-01
dc.description.abstractMuscarinic receptors are the predominant cholinergic receptors in the central and peripheral nervous systems. Recently, activation of muscarinic receptors was found to elicit pigment granule dispersion in retinal pigment epithelium isolated from bluegill fish. Pigment granule movement in retinal pigment epithelium is a light-adaptive mechanism in fish. In the present study, we used pharmacological and molecular approaches to identify the muscarinic receptor subtype and the intracellular signaling pathway involved in the pigment granule dispersion in retinal pigment epithelium. Of the muscarinic receptor subtype-specific antagonists used, only antagonists specific for M1 and M3 muscarinic receptors were found to block carbamyl choline (carbachol)-induced pigment granule dispersion. A phospholipase C inhibitor also blocked carbachol-induced pigment granule dispersion, and a similar result was obtained when retinal pigment epithelium was incubated with an inositol trisphosphate receptor inhibitor. We isolated M2 and M5 receptor genes from bluegill and studied their expression. Only M5 was found to be expressed in retinal pigment epithelium. Taken together, pharmacological and molecular evidence suggest that activation of an odd subtype of muscarinic receptor, possibly M5, on fish retinal pigment epithelium induces pigment granule dispersion.
dc.description.departmentBiology
dc.formatText
dc.format.extent17 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationPhatarpekar, P. V., Durdan, S. F., Copeland, C. M., Crittenden, E. L., Neece, J. D., & García, D. M. (2005). Molecular and pharmacological characterization of muscarinic receptors in retinal pigment epithelium. Journal of Neurochemistry. 95(5), pp. 1504-1520.
dc.identifier.doihttps://doi.org/10.1111/j.1471-4159.2005.03512.x
dc.identifier.urihttps://hdl.handle.net/10877/6356
dc.language.isoen
dc.publisherInternational Society for Neurochemistry
dc.rights.holder© 2005 International Society for Neurochemistry
dc.sourceJournal of Neurochemistry, (2005). Vol. 95, Issue 5, pp. 1504-1520.
dc.subjectacetylcholine
dc.subjectbluegill
dc.subjectlight-adaptation
dc.subjectmuscarinic receptors
dc.subjectpigment granule movement
dc.subjectretinal pigment epithelium
dc.subjectBiology
dc.titleMolecular and Pharmacological Characterization of Muscarinic Receptors in Retinal Pigment Epithelium: Role in Light-adaptive Pigment Movements
dc.typeArticle

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