Effect of Bacteriophage Infection in Combination with Tobramycin on the Emergence of Resistance in Escherichia coli and Pseudomonas aeruginosa Biofilms

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dc.contributor.authorCoulter, Lindsey B.
dc.contributor.authorMcLean, Robert J. C.
dc.contributor.authorRohde, Rodney E.
dc.contributor.authorAron, Gary M.
dc.date.accessioned2020-04-29T19:13:41Z
dc.date.available2020-04-29T19:13:41Z
dc.date.issued2014-10
dc.description.abstractBacteriophage infection and antibiotics used individually to reduce biofilm mass often result in the emergence of significant levels of phage and antibiotic resistant cells. In contrast, combination therapy in Escherichia coli biofilms employing T4 phage and tobramycin resulted in greater than 99% and 39% reduction in antibiotic and phage resistant cells, respectively. In P. aeruginosa biofilms, combination therapy resulted in a 60% and 99% reduction in antibiotic and PB-1 phage resistant cells, respectively. Although the combined treatment resulted in greater reduction of E. coli CFUs compared to the use of antibiotic alone, infection of P. aeruginosa biofilms with PB-1 in the presence of tobramycin was only as effective in the reduction of CFUs as the use of antibiotic alone. The study demonstrated phage infection in combination with tobramycin can significantly reduce the emergence of antibiotic and phage resistant cells in both E. coli and P. aeruginosa biofilms, however, a reduction in biomass was dependent on the phage-host system.
dc.description.departmentClinical Laboratory Science
dc.formatText
dc.format.extent9 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationCoulter, L. B., McLean, R. J. C., Rohde, R. E., & Aron, G. M.. (2014). Effect of bacteriophage infection in combination with tobramycin on the emergence of resistance in Escherichia coli and pseudomonas aeruginosa biofilms. Viruses, 6(10), pp. 3778-3786.
dc.identifier.doihttps://doi.org/10.3390/v6103778
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/10877/9765
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute
dc.rights.holder© The Authors.
dc.rights.licenseThis work is licensed under a Creative Commons Attribution 4.0 International License.
dc.sourceViruses, 2014, Vol. 6, No. 10, pp. 3778-3786.
dc.subjectbacteriophage
dc.subjectantibiotic
dc.subjectresistance
dc.subjectmixed therapy
dc.subjectbiofilms
dc.subjectClinical Laboratory Science Program
dc.titleEffect of Bacteriophage Infection in Combination with Tobramycin on the Emergence of Resistance in Escherichia coli and Pseudomonas aeruginosa Biofilms
dc.typeArticle

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