The Expression of the Intermediate Filaments Glial Fibrillary Acidic Protein and Keratin in the Visual Pathway of Danio Rerio

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dc.contributor.advisorKoke, Joseph R.
dc.contributor.authorHenry, Kyle Joseph
dc.date.accessioned2009-06-09T13:33:24Z
dc.date.available2012-02-24T10:11:14Z
dc.date.issued2009-05
dc.description.abstractThere is some debate in zebrafish literature in regards of what type of intermediate filament (IF) is expressed in astrocytes of zebrafish optic nerve. Some research groups claim glial fibrillary acidic protein (GFAP) is prominent. Others argue cytokeratin is expressed, but not GFAP. Using Immuno histochemistry, GFAP and cytokeratins present in the zebrafish visual pathway were identified. Purified mouse monoclonal antibodies against GFAP and cytokeratin 18 were used in combination with goat anti-mouse IgG antibodies conjugated to Rhodamine Red-X and Alexa Fluor 488, respectively, to identify the IFs found in the zebrafish visual pathway. Observations of immuhistochemically stained tissues under an Olyumpus Fluoview FV1000 confirmed the presence of cytokeratin in the zebrafish optic nerve, but did not present evidence confirming or denying the presence of GFAP in the zebrafish optic nerve.
dc.description.departmentHonors College
dc.formatText
dc.format.extent25 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationHenry, K. J. (2009). The expression of the intermediate filaments glial fibrillary acidic protein and keratin in the visual pathway of Danio Rerio (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas.
dc.identifier.urihttps://hdl.handle.net/10877/3297
dc.language.isoen
dc.subjectintermediate filaments
dc.subjectGFAP
dc.subjectkeratin
dc.subjectzebrafish
dc.subjectDanio rerio
dc.subjectoptic nerve
dc.subjectimmuno histochemistry
dc.subjectHonors College
dc.titleThe Expression of the Intermediate Filaments Glial Fibrillary Acidic Protein and Keratin in the Visual Pathway of Danio Rerio
thesis.degree.departmentHonors College
thesis.degree.disciplineBiology
thesis.degree.grantorTexas State University-San Marcos
txstate.documenttypeHonors Thesis

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