Discrete Nuclear Structures in Actively Growing Neuroblastoma Cells are Revealed by Antibodies Raised Against Phosphorylated Neurofilament Proteins

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dc.contributor.authorWeigum, Shannon E.
dc.contributor.authorGarcia, Dana M.
dc.contributor.authorChristodoulides, Nicholas
dc.contributor.authorKoke, Joseph R.
dc.contributor.authorRaabe, Timothy D.
dc.date.accessioned2016-11-03T19:52:46Z
dc.date.available2016-11-03T19:52:46Z
dc.date.issued2003-04
dc.description.abstractBackground: Nuclear objects that have in common the property of being recognized by monoclonal antibodies specific for phosphoprotein epitopes and cytoplasmic intermediate filaments (in particular, SMI-31 and RT-97) have been reported in glial and neuronal cells, in situ and in vitro. Since neurofilament and glial filaments are generally considered to be restricted to the cytoplasm, we were interested in exploring the identity of the structures labeled in the nucleus as well as the conditions under which they could be found there. Results: Using confocal microscopy and western analysis techniques, we determined 1) the immunolabeled structures are truly within the nucleus; 2) the phosphoepitope labeled by SMI-31 and RT-97 is not specific to neurofilaments (NFs) and it can be identified on other intermediate filament proteins (IFs) in other cell types; and 3) there is a close relationship between DNA synthesis and the amount of nuclear staining by these antibodies thought to be specific for cytoplasmic proteins. Searches of protein data bases for putative phosphorylation motifs revealed that lamins, NF-H, and GFAP each contain a single tyrosine phosphorylation motif with nearly identical amino acid sequence. Conclusion: We therefore suggest that this sequence may be the epitope recognized by SMI-31 and RT-97 mABs, and that the nuclear structures previously reported and shown here are likely phosphorylated lamin intermediate filaments, while the cytoplasmic labeling revealed by the same mABs indicates phosphorylated NFs in neurons or GFAP in glia.
dc.description.departmentBiology
dc.formatText
dc.format.extent10 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationWeigum, S. E., García, D. M., Raabe, T. D., Christodoulides, N. & Koke, J. R. (2003). Discrete nuclear structures in actively growing neuroblastoma cells are revealed by antibodies raised against phosphorylated neurofilament proteins. BMC Neuroscience, 4(6).
dc.identifier.doihttps://doi.org/10.1186/1471-2202-4-6
dc.identifier.urihttps://hdl.handle.net/10877/6328
dc.language.isoen
dc.publisherBioMed Central
dc.rightsThis is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
dc.rights.holder© 2003 Weigum et al.
dc.sourceBMC Neuroscience, 2003, Vol. 4, No. 6.
dc.subjectneuroblastoma cells
dc.subjectphosphoprotein epitopes
dc.subjectcytoplasmic intermediate filaments
dc.subjectBiology
dc.titleDiscrete Nuclear Structures in Actively Growing Neuroblastoma Cells are Revealed by Antibodies Raised Against Phosphorylated Neurofilament Proteins
dc.typeArticle

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