Differential Gene Expression in Danio rerio during Optic Nerve Regeneration

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dc.contributor.advisorGarcia, Dana M.
dc.contributor.advisorKoke, Joseph R.
dc.contributor.authorSaul, Katherine E.
dc.contributor.committeeMemberDharmasiri, Nihal
dc.contributor.committeeMemberRaabe, Timothy
dc.date.accessioned2012-02-24T10:09:19Z
dc.date.available2012-02-24T10:09:19Z
dc.date.issued2008-08
dc.description.abstractSpinal cord injuries and neurodegenerative diseases in mammals result in a loss of function due to the failure of neurons in the central nervous system (CNS) to survive and regenerate their axons. Unlike mammals, fish and amphibians possess the ability to regenerate their CNS following damage. To gain a better understanding of the factors necessary for successful CNS regeneration, I conducted a temporal analysis of the changes in gene expression in the retina caused by optic nerve injury to identify genes specifically involved in regeneration. Dual color oligonucleotide microarrays were used to compare total RNA harvested from retinas of sham-operated and optic nerve-injured fish at 3, 24 and 168 hours following surgery. Statistical analyses identified 722 genes differentially expressed by at least 1.5-fold at one or more time points, and 142 genes with at least a 2.0-fold difference. Based on microarray fold differences and gene ontology analysis, six genes were selected for further analysis using qRT-PCR. The results of qRT-PCR identified noggin 2, activating transcription factor 3, and beta-tubulin 5 as genes that showed significantly increased expression in the injured fish as compared to sham; therefore, these genes’ products may play an important role in optic nerve regeneration in zebrafish. These results support the hypothesis that an analysis of gene expression between optic nerve injured and sham-operated fish will reveal genes specifically involved in regeneration.
dc.description.departmentBiology
dc.formatText
dc.format.extent57 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationSaul, K. E. (2008). <i>Differential gene expression in danio rerio during optic nerve regeneration</i> (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas.
dc.identifier.urihttps://hdl.handle.net/10877/3144
dc.language.isoen
dc.subjectPTIC nerve regenerationen_US
dc.subjectCentral nervous systemen_US
dc.subjectZebrafishen_US
dc.subjectRegenerationen_US
dc.subjectMicroarrayen_US
dc.subjectGene expressionen_US
dc.subject.classificationBiologyen_US
dc.subject.classificationZoologyen_US
dc.titleDifferential Gene Expression in Danio rerio during Optic Nerve Regenerationen_US
dc.typeThesis
thesis.degree.departmentBiology
thesis.degree.disciplineBiology
thesis.degree.grantorTexas State University--San Marcos
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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