Inhibition of Quorum Signalling and Biofilm Formation in Chromobacterium violaceum in the Presence of Cadmium, Cobalt and Nickel Divalent Cations

Simple item page
dc.contributor.advisorMcLean, Robert JC
dc.contributor.authorThornhill, Starla
dc.contributor.committeeMemberVega, Leticia
dc.contributor.committeeMemberKakirde, Kavita
dc.date.accessioned2018-08-13T18:17:52Z
dc.date.available2018-08-13T18:17:52Z
dc.date.issued2016-05
dc.description.abstractBacteria are single celled organisms capable of acting as a single unit by sensing and responding to population density via a phenomenon called quorum signaling. Quorum signaling regulates a variety of phenotypes including biofilm formation and virulence factor production. In the soil bacterium Chromobacterium violaceum the virulence factor violacein results in a deep purple pigmentation and is one such regulated phenotype. Previously, a number of biological and organic molecules have been described as quorum signaling inhibitors, but to date no metal-based inhibitors have been identified. In this study, we show that quorum sensing is inhibited in C. violaceum when in the presence of sub-lethal concentrations of cadmium based salts. Cobalt and nickel salts have also been indicated as inhibitors in other gram negative species. Inhibitory effects of cadmium divalent cations on C. violaceum were shown in biofilm formation, pigmentation and virulence factor production, as well as transcript levels for genes involved in these processes. This study represents the first description of heavy metal based quorum sensing inhibition in C. violaceum.
dc.description.departmentBiology
dc.formatText
dc.format.extent44 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationThornhill, S. (2016). Inhibition of quorum signalling and biofilm formation in Chromobacterium violaceum in the presence of cadmium, cobalt and nickel divalent cations (Unpublished thesis). Texas State University, San Marcos, Texas.
dc.identifier.urihttps://hdl.handle.net/10877/7485
dc.language.isoen
dc.subjectQuorum signaling
dc.subjectBiofilm
dc.subjectAHL
dc.subjectChromobacterium violaceum
dc.subjectC. Violaceum
dc.subjectQuorum sensing
dc.subjectQuorum sensing inhibition
dc.subjectCadmium
dc.subjectCobalt
dc.subjectNickel
dc.titleInhibition of Quorum Signalling and Biofilm Formation in Chromobacterium violaceum in the Presence of Cadmium, Cobalt and Nickel Divalent Cations
dc.typeThesis
thesis.degree.departmentBiology
thesis.degree.disciplineBiology
thesis.degree.grantorTexas State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
THORNHILL-THESIS-2016.pdf
Size:
8.34 MB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.13 KB
Format:
Plain Text
Description:
Download

Collections

Graduate Theses and Dissertations