Show simple item record

dc.contributor.authorDavid, Wendi M. ( )en_US
dc.date.accessioned2009-08-11T10:06:37Z
dc.date.available2012-02-24T10:07:01Z
dc.date.issued2008-01-01en_US
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/2899
dc.descriptionResearch Enhancement Program Final Reports.
dc.description.abstractThe primary goal of the funded research involved using surface plasmon resonance (SPR) for determining real-time interactions between viral SV40 large T-antigen (T-ag), DNA, and a stream of quadruplex DNA-interactive small molecules. We successfully developed an SPR-based assay for monitoring the ability of T-ag to unwind both normal duplex DNA and quadruplex DNA and characterized the ability of several small molecules to inhibit the helicase activity of T-ag. The ability to monitor unwinding of quadruplex DNA in real-time is an advance for investigating the inhibitory effects of quadruplex-interactive agents and this work was published recently in Cell Biochemistry and Biophysics (Feb. 2009). Our results will aid in future characterization of potential therapeutic approaches involving G-quadruplex interactive small molecules and in understanding the structural basis for selective targeting of different families of G-quadruplex DNA helicases. These results form the basis for a recent NIH ARRA grant in collaboration with UT-Austin. In addition, data generated in this research was presented at poster presentations for three national biochemistry meetings. A total of 4 undergraduate students and 1 graduate student were involved in the research.en_US
dc.formatText
dc.format.extent1 page
dc.format.medium1 file (.pdf)
dc.language.isoen_US
dc.subjectViral SV40en_US
dc.subjectT-antigenen_US
dc.subjectT-agen_US
dc.subjectDNAen_US
dc.subjectAnalytesen_US
dc.subjectPlasmon resonanceen_US
dc.subjectSPRen_US
dc.subject.classificationBiochemistryen_US
dc.titleReal-Time Interactions Between SV40 T-Antigen and a Stream of Analytesen_US
txstate.documenttypeResearch Report


Download

Thumbnail

This item appears in the following Collection(s)

Show simple item record