Show simple item record

dc.contributor.authorZhang, Bao ( )
dc.contributor.authorZhuang, Xiyi ( )
dc.contributor.authorGuo, Liyun ( )
dc.contributor.authorMcLean, Robert J. C. ( )
dc.contributor.authorChu, Weihua ( )
dc.date.accessioned2019-09-06T13:20:01Z
dc.date.available2019-09-06T13:20:01Z
dc.date.issued2019-08
dc.identifier.citationZhang, B., Zhuang, X., Guo, L., McLean, R. J. C., & Chu, W. (2019). Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp. Marine Drugs, 17(9).en_US
dc.identifier.urihttps://digital.library.txstate.edu/handle/10877/8603
dc.description.abstractQuorum quenching (QQ) is a promising alternative infection-control strategy to antibiotics that controls quorum-regulated virulence without killing the pathogens. Aeromonas hydrophila is an opportunistic gram-negative pathogen living in freshwater and marine environments. A. hydrophila possesses an N-acyl homoserine lactone (AHL)-based quorum-sensing (QS) system that regulates virulence, so quorum signal-inactivation (i.e., QQ) may represent a new way to combat A. hydrophila infection. In this study, an AHL lactonase gene, aiiA was cloned from Bacillus sp. strain QSI-1 and expressed in Escherichia coli strain BL21(DE3). The A. hydrophila hexanoyl homoserine lactone (C6-HSL) QS signal molecule was degraded by AiiAQSI-1, which resulted in a decrease of bacterial swimming motility, reduction of extracellular protease and hemolysin virulence factors, and inhibited the biofilm formation of A. hydrophila YJ-1 in a microtiter assay. In cell culture studies, AiiAQSI-1 decreased the ability of A. hydrophila adherence to and internalization by Epithelioma papulosum cyprini (EPC) cells. During in vivo studies, oral administration of AiiAQSI-1 via feed supplementation attenuated A. hydrophila infection in Crucian Carp. Results from this work indicate that feed supplementation with AiiAQSI-1 protein has potential to control A. hydrophila aquaculture disease via QQ.en_US
dc.formatText
dc.format.extent12 pages
dc.format.medium1 file (.pdf)
dc.language.isoen_USen_US
dc.publisherMultidisciplinary Digital Publishing Instituteen_US
dc.sourceMarine Drugs, 2019, Vol. 17, No. 9
dc.subjectAeromonas hydrophilaen_US
dc.subjectCrucian Carp
dc.subjectN-acyl homoserine lactone lactonase
dc.subjectBiofilm
dc.subjectVirulence factors
dc.titleRecombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carpen_US
txstate.documenttypeArticle
dc.identifier.doihttps://doi.org/10.3390/md17090499
txstate.departmentBiology


Download

Thumbnail

This item appears in the following Collection(s)

Show simple item record